Comparative Analysis of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Focusing on Nucleic Acid Removal.
(LNJNbio Polystyrene Microspheres)
In the field of modern biotechnology, microsphere materials are extensively made use of in the extraction and filtration of DNA and RNA as a result of their high particular area, great chemical security and functionalized surface area residential or commercial properties. Amongst them, polystyrene (PS) microspheres and their derived polystyrene carboxyl (CPS) microspheres are just one of both most widely examined and used materials. This article is provided with technical assistance and information analysis by Shanghai Lingjun Biotechnology Co., Ltd., aiming to systematically compare the performance differences of these two types of products in the procedure of nucleic acid extraction, covering key indicators such as their physicochemical homes, surface area alteration ability, binding efficiency and healing price, and illustrate their suitable scenarios with speculative information.
Polystyrene microspheres are homogeneous polymer fragments polymerized from styrene monomers with great thermal stability and mechanical strength. Its surface area is a non-polar framework and generally does not have energetic practical teams. Consequently, when it is directly used for nucleic acid binding, it requires to count on electrostatic adsorption or hydrophobic activity for molecular addiction. Polystyrene carboxyl microspheres present carboxyl useful teams (– COOH) on the basis of PS microspheres, making their surface with the ability of further chemical combining. These carboxyl teams can be covalently bonded to nucleic acid probes, healthy proteins or various other ligands with amino teams through activation systems such as EDC/NHS, consequently accomplishing much more steady molecular addiction. Therefore, from an architectural perspective, CPS microspheres have a lot more advantages in functionalization capacity.
Nucleic acid extraction typically includes actions such as cell lysis, nucleic acid release, nucleic acid binding to solid phase service providers, washing to get rid of impurities and eluting target nucleic acids. In this system, microspheres play a core duty as strong phase service providers. PS microspheres mostly depend on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding effectiveness has to do with 60 ~ 70%, yet the elution efficiency is reduced, just 40 ~ 50%. In contrast, CPS microspheres can not only use electrostatic impacts however additionally attain more solid fixation through covalent bonding, lowering the loss of nucleic acids during the cleaning process. Its binding efficiency can get to 85 ~ 95%, and the elution performance is also increased to 70 ~ 80%. Furthermore, CPS microspheres are also substantially better than PS microspheres in regards to anti-interference capacity and reusability.
In order to verify the efficiency differences in between the two microspheres in real operation, Shanghai Lingjun Biotechnology Co., Ltd. carried out RNA extraction experiments. The speculative samples were stemmed from HEK293 cells. After pretreatment with basic Tris-HCl buffer and proteinase K, 5 mg/mL PS and CPS microspheres were utilized for removal. The results showed that the ordinary RNA yield removed by PS microspheres was 85 ng/ μL, the A260/A280 ratio was 1.82, and the RIN value was 7.2, while the RNA yield of CPS microspheres was enhanced to 132 ng/ μL, the A260/A280 proportion was close to the optimal worth of 1.91, and the RIN value reached 8.1. Although the operation time of CPS microspheres is a little longer (28 mins vs. 25 mins) and the cost is higher (28 yuan vs. 18 yuan/time), its extraction quality is dramatically improved, and it is more suitable for high-sensitivity detection, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the point of view of application scenarios, PS microspheres appropriate for large screening projects and initial enrichment with reduced needs for binding uniqueness due to their affordable and simple procedure. Nevertheless, their nucleic acid binding ability is weak and conveniently impacted by salt ion focus, making them inappropriate for long-lasting storage or repeated usage. In contrast, CPS microspheres appropriate for trace sample extraction as a result of their abundant surface practical teams, which assist in additional functionalization and can be utilized to create magnetic bead detection packages and automated nucleic acid removal platforms. Although its preparation procedure is fairly complicated and the cost is fairly high, it reveals more powerful adaptability in scientific study and professional applications with rigorous needs on nucleic acid removal performance and purity.
With the rapid advancement of molecular medical diagnosis, genetics modifying, fluid biopsy and various other fields, greater needs are put on the performance, pureness and automation of nucleic acid extraction. Polystyrene carboxyl microspheres are gradually replacing typical PS microspheres as a result of their superb binding performance and functionalizable features, coming to be the core option of a new generation of nucleic acid extraction products. Shanghai Lingjun Biotechnology Co., Ltd. is additionally continuously maximizing the bit dimension circulation, surface area density and functionalization performance of CPS microspheres and establishing matching magnetic composite microsphere products to satisfy the requirements of professional diagnosis, clinical study establishments and commercial clients for high-grade nucleic acid removal remedies.
Supplier
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need dna preparation, please feel free to contact us at sales01@lingjunbio.com.
All articles and pictures are from the Internet. If there are any copyright issues, please contact us in time to delete.
Inquiry us